Tissue Culture: Start to Finish

Follow these 10 steps to go from a cutting to a potted plant. Written so a high school student can do it.

Equipment Budget Calculator

Include optional equipment

Beginner Plant Protocols

8 species with complete media recipes, growth regulators, success rates, and practical tips.

Standard Operating Procedures

10 print-ready SOPs. Click any SOP to view, print, or export as text.

Sterilization Protocol Calculator

Tissue Type Your Bleach NaOCl % High endophyte risk (plant was outdoors) Use Tween-20 / dish soap

Protocol Result

Click "Calculate Protocol" to generate.

Autoclave Calculator

Media per Vessel (mL)

Number of Vessels

Vessel Type

Include sterile water for rinsing Include tools (scalpel, forceps)

Troubleshooting Guide

Click any problem to expand causes, solutions, and prevention.

Select Media Formulation

Base Medium

Formulation Components

Component	Formula	mg/L	Category

Recipe Configuration

Base Medium

Strength

Volume (L)

Sucrose (g/L)

Agar (g/L)

Target pH Growth Regulator 1

Growth Regulator 2

Preparation Instructions

Recipe

Plant Growth Regulators Database

Abbreviation	Full Name	Type	MW (g/mol)	Solvent	Heat Stable	Range (mg/L)

Stock Solutions

Stock A

Stock B

Dilution Factor

Target EC (mS/cm)

Mix Result

Dilution Ratios & Concentrations

EC to PPM Converter

EC (mS/cm)

Scale

PPM to EC Converter

PPM

Scale

pH Adjustment Calculator

Current pH

Target pH

Volume (L)

Buffer Capacity

Adjustment Recommendation

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Hydroponic Fertilizer Database (11 nutrients)

Name	Formula	N%	P%	K%	Ca%	Mg%	S%	Fe%	EC/g	Solubility